Vergleich

SMCC Activated B-PE (B-Phycoerythrin) - 1 mg

ArtNr AS-72109
Hersteller AnaSpec
Menge 1 mg
Kategorie
Typ Fluorescent dyes and probes
Format In solution
Applikationen FC, IF
Specific against other
Citations Chen FT and Pentoney SL Jr (1994). Characterization of digoxigenin-labeled B-phycoerythrin by capillary electrophoresis with laser-induced fluorescence. Application to homogeneous digoxin immunoassay. J Chromatogr A 680, 425-30. Ficner R, et al. (1992). Isolation, crystallization, crystal structure analysis and refinement of B-phycoerythrin from the red alga Porphyridium sordidum at 2.2 Å resolution. J Mol Biol 228, 935-50.
ECLASS 10.1 30196090
ECLASS 11.0 30196090
UNSPSC 12000000
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Labeled proteins
Manufacturer - Applications
Flow Cytometry; Cell staining; Immunofluorescence (IF)
Manufacturer - Category
Labeling & detection
Manufacturer - Targets
Phycoerythrin
Shipping Temperature
4 °C
Storage Conditions
Store at 4 °C and protect from light. DO NOT FREEZE.
Description
B-Phycoerythrin (B-PE), a fluorescent protein from phycobiliprotein family, is isolated from cyanobacteria and eukaryotic algae. SMCC activated B-PE is chemically modified with SMCC. SMCC reacts with the primary amine on B-PE and introduces maleimide groups to B-PE. These maleimide groups easily react with thiol groups of target protein without the need for any additional activation, resulting in convenient conjugation of B-PE with proteins. Its primary absorption peak is at 545 nm with secondary peak at 563 nm. B-PE and the closely related R-PE are the most intensely fluorescent phycobiliproteins having orange fluorescence. They are significantly brighter and more photostable than conventional organic fluorophores. B-PE conjugates have been widely used in applications such as flow cytometry and multi-color immunofluorescent staining.
Product Group
PA3ZZZ
Research Area
Peptide & Protein Analysis / In solution
Absorbtion (nm)
545
Emission (nm)
578
GTIN
5400535131609
Colour
Orange
Concentration
1 mg/mL
Storage Buffer
MES buffer, pH 6.0 with EDTA and preservative
Detection Method
Fluorescent
Usage
Research use
UNSPSC
12171501

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 mg
Lieferbar: In stock
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