C-PC (C-Phycocyanin) - 1 mg

Flow Cytometry; Cell staining; Immunofluorescence (IF)

Phycocyanin

Store at 4°C and protect from light. DO NOT FREEZE

C-Phycocyanin (C-PC)1 occurs as the major phycobiliprotein in many cyanobacteria and as a secondary phycobiliprotein in some red algae. The pigment has a single visible absorption maximum at 620 nm and a fluorescence emission maximum at ca.642 nm. It shows two bands corresponding to alpha (21, 360 Da) and beta (18, 980 Da) subunits on SDS-PAGE2. These two subunits usually occur in equal numbers, but the exact number of alpha and beta pairs may vary among the species.In addition to absorbing light directly, this intensely blue pigment accepts quanta from phycoerythrin (Cat# AS-82001 and Cat# AS-82004) by fluorescent energy transfer in organisms in which PE is present. The red fluorescence of C-PC can be further transferred to allophycocyanin (Cat# AS-82000).The molar extinction coefficient of C-PC at 620 nm is 1.54 x 106 cm-1M-1. C-PC is supplied in sodium phosphate buffer, pH 7.0, with ammonium sulfate. The protein is very stable and can be stored for years in this buffer.Before use, centrifuge the C-PC suspension at 10, 000 g for 10 min at 4 C. Discard the supernatant and resuspend the pellet into the desired buffer. Store C-PC and C-PC related conjugates at 4 C and keep away from light.

Peptide & Protein Analysis / In solution

620

5400535127510

Sodium phosphate buffer, pH 7.0, with ammonium sulfate

Research use