Collagen (Type I), FITC conjugated Water Insoluble - 5 mg

Protease activity

Matrix Metalloproteinase (MMP); MMP-1

Store at -20°C and protect from light and moisture.

Besides water-soluble collagen (Type I) conjugate (Cat#85111), AnaSpec also offers water-insoluble fluoresceinated collagen (type I). It can also be used for fluorometric measurement of collagenase activity. Cat#85102 is the water-insolulbe collagen that is heavily labeled with FITC, resulting in almost total quenching of the conjugates fluorescence. Protease-catalyzed hydrolysis slowly yields brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. Although this fluoresceinated collagen is more slowly digested by MMP-1 than water-soluble collagen (Cat#85111), it is more specific for the enzyme.

Cell Signaling / Lyophilized

520

Fluorescent

12352202