Goat anti-PTBP1 / PTB Antibody

Goat anti-PTBP1 / PTB Antibody
Principle Name: PTBP1 / PTB
Official Symbol: PTBP1
All Name and Symbols: PTBP1; PTB; polypyrimidine tract binding protein 1; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; HNRNPI; RNA binding protein; heterogeneous nuclear ribonucleoprotein polypeptide I; polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I);
Accession Number(s): NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1
Peptide Sequence: DGIVPDIAVGTKR, is from N Terminus
Human Gene ID(s):
Non-Human GeneID(s): 19205 (mouse); 29497 (rat);
Important Comments: This antibody is expected to recognise all 4 reported isoforms (represented by NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human, Mouse, Rat
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 55kDa band observed in Human, Mouse and Rat Ovary lysates and 60kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat, HEK293 and 3T3N (calculated MW of 57.2kDa according to NP_114368.1). Recommended concentration: 0
Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear membrane staining in all the convoluted tubules. Recommended concentration: 2-4ug/ml.
Reference(s): Charlet-B N, Logan P, Singh G, Cooper TA. Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing. Mol Cell. 2002 Mar; 9(3):649-58..PMID: 11931771