Goat anti-MURF1 / TRIM63 (internal) Antibody

Goat anti-MURF1 / TRIM63 (internal) Antibody
Principle Name: MURF1 / TRIM63 (internal)
Official Symbol: TRIM63
All Name and Symbols: TRIM63; tripartite motif-containing 63 ; HGNC:16007; FLJ32380; IRF; MURF1; MURF2; RNF28; SMRZ ; iris ring finger protein; muscle specific ring finger protein 1; muscle specific ring finger protein 2; ring finger protein 28; striated muscle RING zinc finge
Accession Number(s): NP_115977.2
Peptide Sequence: QQYQEQLDKSTKLVE, is from internal region
Human Gene ID(s): 84676
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments gave an approx 75kDa band in Human Heart and Skeletal Muscle lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calc
Immunohistochemistry: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 1.25ug/ml.
Reference(s): Cao PR, Kim HJ, Lecker SH. Ubiquitin-protein ligases in muscle wasting. Int J Biochem Cell Biol. 2005 Oct; 37(10):2088-97. Epub 2004 Dec 14. .PMID: 16125112