Description |
Goat anti-TRAF1 Antibody Principle Name: TRAF1 Official Symbol: TRAF1 All Name and Symbols: TRAF1; TNF receptor-associated factor 1 ; EBI6; MGC:10353 ; Epstein-Bar virus-induced protein 6 Accession Number(s): NP_005649.1 Peptide Sequence: KLQSPKHAYVKDD, is from C Terminus Human Gene ID(s): 7185 Non-Human GeneID(s): 22029 (mouse); Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB, IHC Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 16000. Western Blot: Approx 50-55kDa band observed in lysates of monocytic leukemia cell line U937 (calculated MW of 46.2kDa according to NP_005649.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pryhu Immunohistochemistry: Paraffin embedded Human Thymus and Small Intestine. Recommended concentration: 2.5ug/ml. Reference(s): Su X, Li S, Meng M, Qian W, Xie W, Chen D, Zhai Z, Shu HB. TNF receptor-associated factor-1 (TRAF1) negatively regulates Toll/IL-1 receptor domain-containing adaptor inducing IFN-beta (TRIF)-mediated signaling. Eur J Immunol. 2006 Jan; 36(1):199-206. .PMID: 16323247 |