Goat anti-Karyopherin (importin) beta 1 Antibody

Goat anti-Karyopherin (importin) beta 1 Antibody
Principle Name: Karyopherin (importin) beta 1
Official Symbol: KPNB1
All Name and Symbols: KPNB1; karyopherin (importin) beta 1; HGNC:6400; IMB1; IPOB; Impnb; MGC2155; MGC2156; MGC2157; NTF97; importin 90; importin beta-1 subunit; karyopherin beta 1; nuclear factor p97; KapB1; IPO1
Accession Number(s): NP_002256.2
Peptide Sequence: RRSKTNKAKTLAT, is from C Terminus
Human Gene ID(s): 3837
Non-Human GeneID(s): 16211 (mouse); 24917 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 90-100kDa band observed in lysates of cell lines Daudi, HEK293, and Jurkat (calculated MW of 97.2kDa according to NP_002256.2). Recommended concentration: 0.03-0.1ug/ml.
Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5ug/ml.
Reference(s): Caudron M, Bunt G, Bastiaens P, Karsenti E. Spatial coordination of spindle assembly by chromosome-mediated signaling gradients. Science. 2005 Aug 26; 309(5739):1373-6. .PMID: 16123300