Antibodies

Goat anti-PIWI / HIWI Antibody

Hersteller Angio-Proteomie
Kategorie
Typ Antibody
Specific against other
Menge 100ug
ArtNr ANG-dAP-1137-100ug
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Description
Goat anti-PIWI / HIWI Antibody
Principle Name: PIWI / HIWI
Official Symbol: PIWIL1
All Name and Symbols: PIWIL1; HIWI; PIWI; piwi-like 1 (Drosophila); piwi (Drosophila)-like 1; homolog of Drosophila piwi; MIWI; piwi homolog; piwi-like 1
Accession Number(s): NP_004755.2; NP_001177900.1
Peptide Sequence: SNRKDKYDAIKKY, is from internal region
Human Gene ID(s): 9271
Non-Human GeneID(s): 57749 (mouse);
Important Comments: This antibody is expected to recognise both reported isoforms (NP_004755.2; NP_001177900.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Preliminary experiments showed a 100kDa band but also bands at approx 35kDa and 26-28kDa in Human Ovary, Placenta, Prostate and Testis lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the
Immunohistochemistry: In paraffin embedded Human Testis shows textured cytoplasm staining in all germ cells. Recommended concentration, 4-6ug/ml.
Reference(s): Szakmary A, Cox DN, Wang Z, Lin H. Regulatory Relationship among piwi, pumilio, and bag-of-marbles in Drosophila Germline Stem Cell Self-Renewal and Differentiation. Curr Biol. 2005 Jan 26; 15(2):171-8. .PMID: 15668175

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Menge: 100ug
Lieferbar: In stock
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