Goat anti-APOB Antibody

Pep ELISA, WB, IHC

Goat anti-APOB Antibody
Principle Name: APOB
Official Symbol: APOB
All Name and Symbols: APOB; apolipoprotein B (including Ag(x) antigen); FLDB; apoB-100; apoB-48; apolipoprotein B; apolipoprotein B48
Accession Number(s): NP_000375.2
Peptide Sequence: TDLHLRYQKDKK, is from internal region
Human Gene ID(s): 338
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Bands corresponding to the expected size of ApoB100 were detected in Human serum samples from five different individuals. Recommended concentration: 0.3-1ug/ml.
Immunohistochemistry: Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 2ug/ml.
Reference(s): Benn M, Nordestgaard BG, Jensen JS, Tybjaerg-Hansen A. Polymorphisms in apolipoprotein B and risk of ischemic stroke. J Clin Endocrinol Metab. 2007 Sep; 92(9):3611-7.PMID: 17595251