Goat anti-ATG4D Antibody

Pep ELISA, IHC

Goat anti-ATG4D Antibody
Principle Name: ATG4D
Official Symbol: ATG4D
All Name and Symbols: ATG4D; ATG4 autophagy related 4 homolog D (S. cerevisiae); APG4-D; APG4D; AUTL4; APG4 autophagy 4 homolog D; AUT-like 4, cysteine endopeptidase; autophagin-4; autophagy-related cysteine endopeptidase 4; cysteine protease involved in autophagy
Accession Number(s): NP_116274.3
Peptide Sequence: CSQLAQPTLRLPRT, is from internal region (near C terminus)
Human Gene ID(s): 84971
Non-Human GeneID(s): 235040 (mouse); 686505 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments gave an approx. 26kDa band in Human Skeletal Muscle lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size
Immunohistochemistry: Paraffin embedded Human Pancreas. Recommended concentration: 2.5ug/ml.
Reference(s): Mariño G, Uria JA, Puente XS, Quesada V, Bordallo J, Lopez-Otin C. Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy. The Journal of biological chemistry 2003 Feb 278 (6): 3671-8.PMID: 12446702