Goat anti-tropomyosin 4 / TPM4 Antibody

Pep ELISA, WB

Goat anti-tropomyosin 4 / TPM4 Antibody
Principle Name: tropomyosin 4 / TPM4
Official Symbol: TPM4
All Name and Symbols: TPM4; tropomyosin 4; TM30p1; tropomyosin alpha-4 chain; tropomyosin-4
Accession Number(s): NP_001138632.1; NP_003281.1
Peptide Sequence: ENVGLHQTLDQ, is from C Terminus
Human Gene ID(s): 7171
Non-Human GeneID(s): 326618 (mouse); 24852 (rat);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001138632.1; NP_003281.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 32kDa band observed in Human Platelets, Pig Heart and in NIH3T3 lysates (calculated MW of 32.7kDa according to NP_001138632.1). Recommended concentration: 0.1-0.3ug/ml.
Reference(s): Vlahovich N, Schevzov G, Nair-Shaliker V, Ilkovski B, Artap ST, Joya JE, Kee AJ, North KN, Gunning PW, Hardeman EC. Tropomyosin 4 defines novel filaments in skeletal muscle associated with muscle remodelling/regeneration in normal and diseased muscle. Cell motility and the cytoskeleton 2008 Jan 65 (1): 73-85.PMID: 17968984