Goat anti-Robo3.2 (mouse aa1303-1316) Antibody

Goat anti-Robo3.2 (mouse aa1303-1316) Antibody
Principle Name: Robo3.2 (mouse aa1303-1316)
Official Symbol: Robo3
All Name and Symbols: Robo3; roundabout homolog 3 (Drosophila); Rbig1; Rig-1; Rig1; Robo3a; Robo3b; retinoblastoma inhibiting gene 1; retinoblastoma-inhibiting gene 1 protein; roundabout homolog 3
Accession Number(s): AAD11628.1
Peptide Sequence: KESQGRGRGLEACR, is from C-terminus
Human Gene ID(s):
Non-Human GeneID(s): 19649 (mouse);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Preliminary experiments gave bands at approx 85kDa in Mouse fetal Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of
Reference(s): Kuwako K, Kakumoto K, Imai T, Igarashi M, Hamakubo T, Sakakibara S, Tessier-Lavigne M, Okano HJ, Okano H. Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression. Neuron. 2010 Aug 12; 67(3):407-21..PMID: 20696379