Description |
Product Name: Granulocyte Macrophage-Colony Stimulating Factor Rhesus Macaque Recombinant Synonyms: CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897. Description: Granulocyte Macrophage Colony Stimulating Factor Monkey Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14.4 kDa.GM-CSF is purified by proprietary chromatographic techniques. Uniprot Accesion Number: Q0MUT8 Amino Acid Sequence:APARSPSPGT QPWEHVNAIQ EARRLLNLSR DTAAEMNKTV EVVSEMFDLQ EPSCLQTRLE LYKQGLQGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFQSFKENL KDFLLVIPFD CWEPVQE. Source: Escherichia Coli. Physical Appearance and Stability: Lyophilized GMCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution GMCSF should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. Formulation and Purity: GM-CSF was lyophilized from a concentrated (1mg/ml) solution containing 1x PBS pH 7.4. Greater than 98.0% as determined by SDS-PAGE and RP-HPLC. Application: Solubility: It is recommended to reconstitute the lyophilized GMCSF in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1ng/ml, corresponding to a specific activity of > 10, 000, 000 IU/mg. Shipping Format and Condition: Lyophilized powder at room temperature. Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |