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MyoD1(5.8A + MYD712), CF740 conjugate, 0.1mg/mL

ArtNr B-BNC740713-100
Hersteller Biotium
Menge 100 uL
Kategorie
Typ Antibody Monoclonal
Clon 5.8A MYD712
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Chicken (Gallus gallus domesticus)
Host Mouse
Isotype IgG
Citations Thulasi R et. al. Cell Growth and Differentiation, 1996, 7(4):531-41. | Wesche WA et. al. American Journal of Surgical Pathology, 1995, 19(3):261-9. | Parham DM et. al. Acta Neuropathologica, 1994, 87:605-11
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias bHLHc1,Class C basic helix-loop-helix protein 1,Myoblast determination protein 1,Myogenic differentiation 1,Myogenic factor 3 (Myf-3),Myogenin D1,PUM
Versandbedingung Raumtemperatur
Lieferbar
Manufacturer - Type
Primary
Manufacturer - Category
Primary Antibodies Only
Manufacturer - Isotype
IgG' s
Manufacturer - Targets
MyoD1
Manufacturer - Conjugate / Tag
CF740
Manufacturer - Host
Mus musculus (mouse), Bos taurus (bovine)
Shipping Temperature
Room Temperature (Next day)
Storage Conditions
Store at 2 to 8°C|Protect fluorescent conjugates from light
Molecular Weight
45 kDa
Manufacturer - Research Area
Cancer|Developmental biology
Description
Recognizes a phosphor-protein of 45 kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin
Animal
Ingredient of biological origin
Mus musculus (mouse), Bos taurus (bovine)
Special handling for PI and label
Protect from light
Undated stability guarantee in PI
2 years
Stability at RT during shipping (protected from light)
Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity
41116161
UNSPSC Commodity Title
Primary and secondary antibodies for multiple methodologyimmunostaining detection application
Classified or regulated chemicals
0.05% sodium azide (CAS 26628-22-8)
Concentration
0.1 mg/mL
Storage buffer
PBS, 0.1% rBSA, 0.05% azide
Dated shelf life printed on label
Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)
Recombinant mouse MyoD1 protein (5.8A); Recombinant human MyoD1 protein (MYD712)
Clonality
Monoclonal
Regulatory Status
For research use only (RUO)
Human Gene Symbol
MYOD1
Unigene
181768
Positive Control
Rhabdomyosarcoma
Antibody target cellular localization
Nucleus
Cell tissue expression
Muscle
Tumor expression
Sarcoma
Antibody application notes
For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry|Only nuclear staining should be considered as evidence of skeletal muscle differentiation
Manufacturer - Antibody Reactivity
MyoD1
Antibody number
#0713

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 uL
Lieferbar: In stock
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