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CD30(Ki-1/779), CF740 conjugate, 0.1mg/mL

ArtNr B-BNC740779-100
Hersteller Biotium
Menge 100 uL
Kategorie
Typ Antibody Monoclonal
Applikationen IHC
Clon Ki-1/779
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 Kappa
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias CD30L receptor,Cytokine receptor CD30,Ki-1 antigen,Lymphocyte activation antigen CD30,Tumor necrosis factor receptor superfamily member 8 (TNFRSF8)
Versandbedingung Raumtemperatur
Lieferbar
Manufacturer - Type
Primary
Manufacturer - Applications
IHC, FFPE (verified)
Manufacturer - Category
Primary Antibodies Only
Manufacturer - Targets
CD30|TNFRSF8
Manufacturer - Conjugate / Tag
CF740
Manufacturer - Host
Mus musculus (mouse), Bos taurus (bovine)
Shipping Temperature
Room Temperature (Next day)
Storage Conditions
Store at 2 to 8°C|Protect fluorescent conjugates from light
Molecular Weight
105-120 kDa
Manufacturer - Research Area
Immunology
Description
Recognizes a single chain glycoprotein of 105/120 kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90 kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120 kDa glycoprotein. In Hodgkin's disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This MAb distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin
Animal
Ingredient of biological origin
Mus musculus (mouse), Bos taurus (bovine)
Special handling for PI and label
Protect from light
Undated stability guarantee in PI
2 years
Stability at RT during shipping (protected from light)
Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity
41116161
UNSPSC Commodity Title
Primary and secondary antibodies for multiple methodologyimmunostaining detection application
Classified or regulated chemicals
0.05% sodium azide (CAS 26628-22-8)
Concentration
0.1 mg/mL
Storage buffer
PBS, 0.1% rBSA, 0.05% azide
Dated shelf life printed on label
Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)
Recombinant human TNFRSF8 protein
Clonality
Monoclonal
Regulatory Status
For research use only (RUO)
Human Gene Symbol
TNFRSF8
Unigene
1314
Positive Control
Hodgkin's lymphoma
Antibody target cellular localization
Plasma membrane
Cell tissue expression
Lymphocytes
Tumor expression
Leukemia/lymphoma
Antibody applications
IHC, FFPE (verified)
Verified antibody applications
IHC (FFPE) (verified)
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
Manufacturer - Antibody Reactivity
CD30|TNFRSF8
Antibody number
#0779

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 uL
Lieferbar: In stock
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