Vergleich

Anti-VGAT Antibody

ArtNr 75-457
Hersteller Antibodies Incorporated
Menge 100 uL
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, IHC, ICC
Clon L118/80
Specific against Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Mouse
Isotype IgG2a
Konjugat/Tag Unconjugated
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Vesicular inhibitory amino acid transporter (GABA and glycine transporter) (Solute carrier family 32 member 1) (Vesicular GABA transporter) (mVGAT) (mVIAAT)
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Primary Antibody
Manufacturer - Targets
VGAT
Country of Origin
United States
Shipping Temperature
Shipped on ice packs
Storage Conditions
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Molecular Weight
50 kDa
Manufacturer - Research Area
Cell Markers, Neuronal Markers, Synapse Markers, Pre-synaptic Markers, Transporter Proteins
Expiration
24 months from date of receipt
Immunogen
Fusion protein amino acids 1-133 (cytoplasmic N-terminus) of mouse VGAT (accession number O35633) produced recombinantly in E. Coli
Immunogen Species
Mouse
Description
Our Anti-VGAT mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L118/80. It detects mouse and rat VGAT, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Target Description
The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).
Clonality
Monoclonal
Manufacturer - Specificity
No cross-reactivity reported
Concentration
1 mg/mL
Form
Purified by Protein A chromatography
Buffer
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Production Notes
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
Quality Control
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
Quality Control: Application
WB Brain
Dilution Range: WB
1:1000
Dilution Range: IHC
1:500
Usage Statement
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Manufacturer - Statement
We produce our VGAT mouse monoclonal primary antibody from hybridoma clone L118/80. It is great in IHC, ICC, WB and is purified by Protein A chromatography.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 uL
Lieferbar: In stock
lieferbar

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