MALT1 (Paracaspase, Mucosa-associated Lymphoid Tissue Lymphoma Translocation Protein 1) (PE)

Antibodies / Antibodies-Apoptosis

4°C Do Not Freeze

Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with R-Phycoerythrin (PE).

Recombinant protein corresponding to aa701-808 from human MALT1.

Mucosa associated lymphoid tissue lymphoma translocation gene 1 (MALT1) is found in extranodal low-grade B cell lymphomas. MALT1 encodes two Ig-like C2-type domains and fuses with an API2 gene, which is highly expressed in adult lymphoid tissue. The translocation of this MALT1 gene, which maps to human chromosome 18q21, and the apoptosis-inhibiting API2 gene results in an increased development of MALT lymphomas and apoptosis inhibition. Sites at which this API2-MALT1 (11; 18)(q21; q21) translocation commonly occurs are within human lung and kidney tissue. MALT lymphoma expresses nuclear Bcl10, which mediates the oligomerization and activation of a MALT1 caspase-like domain. MALT1 mRNA is found in pre-B cells, mature B cells and plasma cells.

Applications:
Suitable for use in Western Blot, Flow Cytometry (Not Tested), Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry (Not Tested): 0.5-1ug/10e6 cells
Immunofluorescence: 1-2ug/ml
Western Blot: 0.5-1ug/ml
Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT
Immunoprecipitation: 1-2ug/500ug protein lysate
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat, Daudi or HeLa cells, tonsil or lymphoma.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.