IRF8 (Interferon Regulatory Factor 8, ICSBP1)

Antibodies / Antibodies-Interferon

-20°C

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

Recombinant protein corresponding to aa255-426 from human IRF8 expressed in E. coli (UniProt # Q02556).

IRF8 (interferon regulatory factor 8; also ICSBP) is a 50kD member of the IRF family of proteins. It is expressed in myeloid, dendritic and B cells and interacts with PU.1 to regulate the expression of TLR4, IL­18, IL­12p35, and PTPN13, a Fas­associated phosphatase that blocks Fas­induced apoptosis. Human IRF8 is 426 amino acids (aa) in length. It contains an N­terminal DNA­binding basic region (aa9­110) and at least one regulatory phosphorylation site at Tyr95. Over aa255­426, human IRF8 shares 90% and 88% aa sequence identity with canine and mouse IRF8, respectively.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml
Immunohistochemistry: 8­25ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.