MKI67 (Ki-67, KIA, TSG126)

Antibodies / Antibodies-Nuclear Proteins

-20°C

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.

Recombinant protein corresponding to aa3120-3256 form MKI67, expressed in E. coli (UniProt # P46013).

MKI67 (also Ki-67) is a 350-400kD nuclear protein that belongs to a molecular group comprised of mitotic chromosome-associated proteins. Ki-67 was originally recognized as an antigen associated with the monoclonal Ki-67 antibody raised against Hodgkin's lymphoma nuclear material. Ki-67 is contextually expressed, being potentially found in all cells that are not in the Go phase of the cell cycle. Thus, MKI67 qualifies as a cell proliferation marker. Functionally, Ki-67 is known to interact with 160kD Hklp2, a protein that promotes centrosome separation and spindle bipolarity. It also directly interacts with NIFK, and apparently binds to UBF, thus playing a role in rRNA synthesis. Human MKI67 is 3256 amino acids (aa) in length. It contains one FHA domain (aa 8-98), followed by at least 24 utilized Ser/Thr phosphorylation sites and sixteen 120aa repeats (aa 1000-2928) that are interspersed with at least 90 additional utilized phosphorylation sites. There are two potential isoform variants. One isoform is 315-345kD in size and shows a deletion of aa136-495, while a second isoform contains a 58 aa substitution for aa1-513. Over aa3120-3256, human Ki-67 shares 46% aa sequence identity with the mouse ortholog to Ki-67.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Immunocytochemistry: 5-15 ug/ml using immersion fixed A549 human lung carcinoma cells
Immunohistochemistry: 5-15 ug/ml using immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to heat-induced epitope retrieval.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.