Nicalin (Ncl-1, NCLN, NET59, NICAstrin-Like protein)

Antibodies / Antibodies-Proteins

-20°C

Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.

Recombinant protein corresponding to aa1-522 from human Nicalin, S. frugiperda insect ovarian cell line Sf 21-derived UniProt # Q969V3).

Nicalin (NICAstrin-Like proteIN) is a 60-62kD member of the nicastrin family of proteins. It is an ER-embedded transmembrane protein that is found in skeletal and cardiac muscle, hepatocytes and neurons. Nicalin interacts with 22kD TMEM147 and 130kD NOMO1 to form a 200-220kD complex that antagonizes Nodal signaling. Mature human nicalin is a 521 amino acid (aa) type I transmembrane glycoprotein. It contains a 480aa luminal/extracellular region (aa 43-522) plus a 20aa cytoplasmic domain (aa544-563). The luminal region possesses one likely inactive M28 Zn-peptidase-like domain (aa130-429). Four potential isoform variants are reported. One shows an alternative start site at Met75, a second contains a seven aa insertion after Gln543, a third contains a 102aa substitution for aa162-563, and a fourth shows a deletion of Gln446. Over aa1-522, human nicalin shares 95% aa sequence identity with mouse nicalin.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.