IRF8 (Interferon Regulatory Factor 8, IRF-8, Interferon Consensus Sequence-binding Protein, ICSBP, IRF8, ICSBP1, H-ICSBP)

Antibodies / Antibodies-Interferon

-20°C

Supplied as a liquid in PBS, 0.02% sodium azide.

Synthetic peptide corresponding to 16aa from near the C-terminus of human IRF8.

Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. Unlike IRF3, IRF8 appears to act as a negative regulator of IFN-induced genes in most cases, but IRF8 mediates activation of NF-kB by the toll-like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells. Finally, it has been shown that IRF8 decreases bcl-2 expression and thus may play a role in chronic myelogenous leukemia.

Applications:
Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml
Immunofluorescence: 10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Thymus Tissue Lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.