MUC18 (CD146,Cell Surface Glycoprotein MUC18,Cell Surface Glycoprotein P1H12,Gicerin,MCAM,Melanoma Adhesion Molecule,Melanoma Associated Glycoprotein MUC18,Melanoma Cell Adhesion Molecule,Melanoma-associated Antigen A32,

Antibodies / Antibodies-CD Markers

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

Recombinant human MUC18 protein. Cellular Localization: Cell surface

The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.

Applications: 
Suitable for use in Immunofluorescence, Flow Cytometry (Not Tested), FLISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
FLISA: For coating, order antibody without BSA  
Flow Cytometry (Not Tested): 0.5-1ug/million cells 
Immunofluorescence: 0.5-1.0ug/ml 
Immunoprecipitation: 1-2ug/500ug protein
Western Blot: 0.5-1.0ug/ml
Immunohistochemistry: Frozen: 0.5-1.0ug/ml for 30 min at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.

Note: Applications are based on unconjugated antibody.