gp100 (95kD melanocyte-specific Secreted GlycoProtein,M-beta,Melanocyte lineage specific antigen GP100,Melanocyte Protein Pmel 17,Melanoma Associated ME20 antigen,Melanosomal matrix Protein17,p100,p26,PMEL17,Premelanosom

Mab

IgG2b, k

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

Membranes from a human melanoma metastasis. Cellular Localization: Cytoplasmic

By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry (Not Tested), FLISA, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry (Not Tested): 0.5-1ug/million cells
Immunofluorescence: 1-2ug/ml
Immunohistochemistry (Frozen & Formalin-fixed): 1-2ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control:
SK-MEL-28 cells or Melanoma

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.

Note: Applications are based on unconjugated antibody.