gp100 (95kD melanocyte-specific Secreted GlycoProtein,M-beta,Melanocyte lineage specific antigen GP100,Melanocyte Protein Pmel 17,Melanoma Associated ME20 antigen,Melanosomal matrix Protein17,p100,p26,PMEL17,Premelanosom

Mab

IgG2b, k

-20°C

Supplied as a liquid in PBS. Also available with BSA and azide, see 172102.

Membranes from a human melanoma metastasis. Cellular Localization: Cytoplasmic

By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.5-1ug/million cells
Immunofluorescence: 1-2ug/ml
Immunohistochemistry (Frozen & Formalin-fixed): 1-2ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control:
SK-MEL-28 cells or Melanoma

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.