Calbindin (CALB, CALB1, Calbindin 1, (28kD), Calbindin 1, 28kD, Calbindin D28, D-28K, OTTHUMP00000166027, OTTHUMP00000225441, RTVL-H protein, Vitamin D-dependent calcium-binding protein, avian-type)

Antibodies / Antibodies-Proteins

-20°C

Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

Synthetic peptide corresponding to C-KTFVDQYGQRDDGK, from the internal region of the protein sequence according to NP_004920.1. Species sequence homology: rat, canine, porcine and bovine

Calbindin D28 (also termed vitamin D-dependent calcium-binding protein or cholecalcin), is a highly conserved 28kD calcium binding protein, with broad tissue distribution. It belongs, together with calmodulin, S-100, parvalbumin, troponin C and other proteins, to a family of low molecular weight calcium-binding proteins (CaBPs). These CaBPs have homologous primary structures, which contain conserved polypeptide folds of the EF-hand type for Ca2+ binding. Calbindin D28 is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial nervous system neurons, and in certain epithelial cells involved in Ca2+ transport such as distal tubular cells and cortical collecting tubules of the kidney, and in enteric neuroendocrine cells.

Applications:
Suitable for use in Peptide ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Peptide ELISA: 1:128, 000
Western Blot: 0.03-0.1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.