GFAP (Glial Fibrillary Acidic Protein, Astrocyte)

Pab

IgY

-20°C

Supplied as a lyophilized powder in 5% trehalose. Reconstitute with sterile ddH2O.

Recombinant protein corresponding to bovine Glial Fibrillary Acidic Protein expressed in E. coli, and native bovine GFAP.

Glial fibrillary acidic protein (GFAP) is ~50kD intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:50, 000
Immunocytochemistry: 1:1000 using fluorescent secondary antibodies or 1:5000 using peroxidase or other enzyme-linked methods
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.