Smad 1,2,3,5 (Mothers Against Decapentaplegic Homolog,MAD Homolog,Mothers Against DPP Homolog,JV4-1,Mad-Related Protein,SMAD Family Member,SMAD 1,SMAD 2,SMAD 3,SMAD 5,Smad1,Smad2,Smad3,Smad5,hSMAD1,hSMAD2,hSMAD3,hSMAD5,T

Antibodies / Antibodies-Smad Proteins

-20°C

Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg sodium azide. Reconstitute with 100ul sterile ddH2O.

Synthetic peptide corresponding to aa442-456, GPLQWLDKVLTQMGS, from the C-terminal of human Smad 1, 2, 3, 5. Species Sequence Homology: Mouse

Smad proteins are essential mediators of signal transduction by the TGF-beta superfamily from the cell surface into the nucleus. Once in the nucleus, Smad proteins target a number of DNA binding proteins to stimulate transcription.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.