FGFR4 (CD 334, CD334, CD334 antigen, Fc13h 10, Fc13h10, Fgfr 4, FGFR-4, Fgfr4)

Antibodies / Antibodies-Receptors

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide.

Cytoplasmic domain of human FGFR4 fused to maltose binding protein, Swiss ID = P22455

FGFR4 is a member of the fibroblast growth factor receptor family, where aa sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. Although alternative splicing has been observed, there is no evidence that the C-terminal half of the IgIII domain of this protein varies between three alternate forms, as indicated for family members 1-3. This particular family member preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis.

Applications:
Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:100. Detects a band of approximately 105kD (predicted molecular weight: 88kD).
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.