Myelin Basic Protein (MBP)

Pab

IgY

-20°C

Supplied as a liquid, 0.02% sodium azide.

Mixture of 3 MBP peptides which are the different forms of the protein which are alternate transcription from a single gene.

Myelin basic protein is reported to account for about 30 of the proteins in myelin found in the central nervous system. It can induce experimental allergic encephalomyelitis (EAE), a T-lymphocyte mediated disease due to delayed-type hypersensitivity; though each animal species appears to respond to a different fragment of the 170aa polypeptide. Four different isoforms have been identified through cDNA cloning. All four of these variants are identical except for the insertion or deletion of two peptide fragments encoded by exons 2 and 5. Myelin basic protein is reported to be expressed in oligodendrocytes, myelin of white matter in the brain and spinal cord and in peripheral nerves, though it is expressed less abundantly in gray matter.

MBP has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. In vitro, MBP is suitable as a substrate for numerous protein kinases, including the ERK and p38 MAP kinases that phosphorylate MBP at T98. 

Applications:
Suitable for use in Immunofluorescence, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunofluorescence (IC): 1:1000
Western Blot: 1:10, 000
Immunohistochemistry (paraffin): 1:20, 000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.