PARK2 (Juvenile Parkinson Disease Autosomal Recessive 2, Parkinson Disease Autosomal Recessive 2, PARK 2, PARK-2, Parkin) (HRP)

Antibodies / Antibodies-Ubiquitin Ligases

-20°C

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

Residues aa62-80, DQQSIVHIVQRPWRKGQEM, of 52kD human Parkin.

Mutations in the gene, Parkin (PARK2), appear to be responsible for the pathogenesis of autosomal recessive juvenile Parkinsonism. Parkin plays a role in the ubiquitin-mediated proteolytic pathway by removal and/or detoxification of abnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of Parkin.

Applications:
Suitable for use in Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250-1:1000
Immunoprecipitation: 1:250-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.