Beta-Actin

Mab

Blue Ice

Affinity Purified

30021010

b-actin (gene name ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two non-muscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. The b-actin gene is often stably and constitutively expressed at high levels in most tissues and cells. Proteins such as b-actin, involved in maintenance of basic cellular function, are often referred to as housekeeping proteins. Housekeeping proteins are frequently used as loading controls for western blots and protein normalization.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Dot Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:3000
Immunohistochemistry: 1:500-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.