GAPDH (Glyceraldehyde 3-Phosphate Dehydrogenase)

Antibodies / Antibodies-Enzymes, Dehydrogenase

-20°C

Supplied as a lyophilized powder in PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile ddH2O.

Recombinant protein corresponding to GAPDH.

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a 37kD glycolytic enzyme constitutively and stably expressed in almost all tissues. This enzyme catalyzes the sixth step of glycolysis by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Proteins such as GAPDH involved in maintenance of basic cellular function are often referred to as housekeeping protein. Housekeeping proteins are frequently used as loading controls for western blots and protein normalization.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Dot Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:2000
Immunohistochemistry: 1:500-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.