RFP-Tag (Red Fluorescent Protein)

Antibodies / Antibodies-Epitope Tag, RFP

-20°C

Supplied as a lyophilized powder in PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile ddH2O.

Synthetic peptide corresponding to RFP from the Discosoma sea anemone at N-terminal, KLH conjugated.

Red Fluorescent Protein (RFP) is a protein derived from the Discosoma sea anemone. Fluorescent proteins are powerful tools to study protein localization and dynamics in living cells. The anti-RFP antibody can be used to detect native RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange and tdTomato.

Applications:
Suitable for use in Immunohistochemistry, Dot Blot, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:500-1:2000
Western Blot: 1:1000-1:3000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.