S-Adenosylmethionine (SAM)

Antibodies / Antibodies-Enzymes

-20°C

Supplied as a liquid in PBS, pH 7.4, 10mg/ml BSA, 0.02% sodium azide.

S-adenosylmethionine analog, conjugated KLH. Cellular Localization: Cytoplasm, nuclear

S-Adenosylmethionine (SAM-e, SAMe, SAM, AdoMet, ademetionine) was discovered in Italy by G. L. Cantoni in 1952. It is made from Adenosine Triphosphate (ATP) and Methionone by Methionine Adenosyltransferase. S-adenosylmethionine is a critical metabolite in most living organisms. It performs important functions of transmethylation, transsulfuration, and aminopropylation through decarboxylation.

Since SAM is an intrinsically unstable molecufle, and its optical density maximum of 258-260 nm is not a distinguished absorption, the determination of its concentration in various biological fluids and tissues has always been a challenging task. A simple, convenient method that does not require costly instrumentation (LC, MS, and combinations) is clearly desirable for the determination of the biological concentration of SAM, and to monitor its change and metabolic paths in the body fluids, tissues and organelles.

SAM-e is claimed to be helpful for the following conditions: Depression, Parkinson's Disease, Osteoarthritis symptoms, Liver disease, Intrahepatic Cholestasis (of pregnancy especially), Fibormyalgia, AIDS-related myelopathy (a spine and nerve disorder associated with AIDS). There has be also limited research that has referenced for SAMe's role in the following conditions: Attention deficit hyperactivity disorder (ADHD), Premenstrual syndrome (PMS), Premenstrual dysphoric disorder (PMDD), Chronic fatigue syndrome (CFS), heart disease, defective intellectual performance, multiple sclerosis, dementia, Alzeimer's disease, tendonitis, bursitis, spinal cord injury, seizure, migraine headache, chronic low back pain, lead poisoning and aging.

It is also critically necessary to understand the impact and consequence when SAM is being used daily as a nutritional supplement these days. It has been widely used as over-ther-counter medicine to relieve systems of depression, osteoarthristis and so on.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Immunohistochemistry, Dot Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:20-1:40
ELISA: 1:2000-1:3000
Immunohistochemistry: 1:20-1:40
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.