ALOX5 (Arachidonate 5-Lipoxygenase,5 LO,5 LOX,5-Lipoxygenase,5-LO,5-LOX,5LOX,5LPG,ALOX 5,Alox5,Arachidonate 5 Lipoxygenase,Arachidonate 5-Lipoxygenase,Arachidonic 5-Lipoxygenase alpha-10 Isoform,Arachidonic 5-Lipoxygenas

Antibodies / Antibodies-Leukotrienes

-20°C

Supplied as a lyophilized powder in 0.2mg Na2HPO4, 5mg BSA, 0.9mg sodium chloride, 0.05mg sodium azide. Reconstitute with 200ul sterile dH2O to 500ug/ml.

Recombinant protein corresponding to human ALOX5 expressed in E.coli. (Position: A120-R483). Species sequence homology: mouse and rat (94%)

Arachidonate 5-lipoxygenase, also known as 5-LOX or 5-LO, is an enzyme that in humans is encoded by the ALOX5 gene. ALOX5 is a member of the lipoxygenase family of enzymes. It is mapped to 10q11.21. ALOX5 plays a dual role in the synthesis of leukotrienes from arachidonic acid. The position of ALOX5 within the nucleus of resting cells determines the capacity to generate LTB4 upon subsequent activation. It is involved in lung vascular tone regulation and in the development of chronic pulmonary hypertension in hypoxic rodent models. ALOX5 also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.