AHNAK (Desmoyokin, AHNAKRS, AHNAK Nucleoprotein, Neuroblast differentiation-associated protein AHNAK)

Antibodies / Antibodies-Nuclear Proteins

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Met1-Asp300 of human AHNAK, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.

The first of these genes identified encoded the cell surface protein CD44, an integral membrane glycoprotein that is the principal receptor for hyaluronate on the cell surface. A second gene, originally designated PM227, attracted their attention because its expression appeared to be coordinated with that of CD44. The amino acid sequence of AHNAK suggested secondary structure with a periodicity of 2.33 residues per turn. Individual chains could associate to form a 7- or 8-stranded barrel. The resulting structure would be a polyionic rod with length as great as 1.2 microns. Preliminary evidence indicated that the protein resides predominantly within the nucleus, but no function had been discerned. The highly conserved repeated elements were, for the most part, 128aa long.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.