CUL9 (Cullin 9, PARC, H7AP1, P53-Associated Parkin-Like Cytoplasmic Protein, UbcH7-associated protein 1)

Antibodies / Antibodies-Cullin

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Ile1144-Ala1322 of human CUL9, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.

PARC directly interacted and formed an approximately 1-MD complex with p53 in the cytoplasm of unstressed cells. In the absence of stress, inactivation of PARC induced nuclear localization of endogenous p53 and activated p53-dependent apoptosis. Overexpression of PARC promoted cytoplasmic sequestration of ectopic p53. Abnormal cytoplasmic localization of p53 was observed in a number of neuroblastoma cell lines; RNA interference-mediated reduction of endogenous PARC significantly sensitized these neuroblastoma cells in the DNA damage response. These results revealed that PARC is a critical regulator in controlling p53 subcellular localization and subsequent function.PARC also has a unique motif that is highly homologous to the C terminus of cullin proteins that the authors referred to as the C-terminal cullin homology domain.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunocytochemistry: 1:50-500
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.