FIL1d (Interleukin 1 Delta, IL1HY1, IL36RN, IL36Ra, IL1RP3, IL1L1, IL1F5, Interleukin 1 Family, Member 5, Interleukin-1 HY1, IL-1 Related Protein 3, Interleukin 36 Receptor Antagonist)

Antibodies / Antibodies-Interleukin

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Lys12-Phe151 of human FIL1d, expressed in E. coli.

PCR analysis revealed expression in leukocytes, spleen, and brain as well as in fetal brain and most abundantly in a fetal skin library. RT-PCR analysis also established that IL1HY1 expression is amplified in a stimulated macrophage cell line. By EST database searching, Smith et al. (2000) also identified a cDNA encoding IL1HY1, which they termed FIL1-delta. Modeling indicated that IL1HY1 has a conserved 12-stranded beta-trefoil structure. By searching EST databases for IL1-like ligands, Busfield et al. (2000) also cloned IL1HY1, which they designated IL1RP3. SDS-PAGE analysis indicated that IL1HY1 is expressed as a 23kD protein. Northern blot analysis detected expression of a 2.8-kb transcript in placenta and uterus. In situ hybridization analysis revealed constitutive expression by keratinocytes in vitro.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.01-2ug/ml
Immunohistochemistry (Paraffin): 5-20ug/ml
Immunocytochemistry: 5-20ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.