IRF8 (Interferon Regulatory Factor 8, H-ICSBP, ICS-BP, ICSBP1,Interferon Consensus Sequence-Binding Protein 1)

Antibodies / Antibodies-Interferon

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Gly128-Val426 of human IRF8, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.

Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-Beta. IRF family proteins also control expression of IFN-alpha and IFN-Beta-regulated genes that are induced by viral infection. Mouse IFN-producing cells (mIPCs) were absent in all lymphoid organs from ICSBP KO mice, as revealed by lack of CD11c(low)B220(+)Ly6C(+)CD11b(-) cells.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.