MFN1 (Mitofusin 1,Hfzo1, Hfzo2, Transmembrane GTPase MFN1, Fzo homolog)

Antibodies / Antibodies-GTPase, Rab, Ras, Rho Proteins

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Met1-Ile234 of mouse MFN1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.

Mitofusin-1 is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. The deduced 741aa protein contains an N-terminal GTPase domain and a transmembrane domain near the C terminus. MFN1 shares 60% identity with MFN2, with the most extensive homology in the GTPase domains. The N- and C-terminal ends are more variable. When stably expressed in COS-7 cells, MFN1 colocalized with mitochondrial markers. Overexpression of MFN1 caused perinuclear mitochondrial clustering.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.