NBL1 (Neuroblastoma, Suppression Of Tumorigenicity 1, NB, DAN, NO3, DAND1, Differential Screening-Selected Gene Aberrant In Neuroblastoma, DAN domain family member 1)

Antibodies / Antibodies-Cancer Markers

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Ala17-Asp181 of human NBL1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.

NBL1 is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.Enomoto et al. (1994) isolated the human DAN counterpart from a normal lung cDNA library by using rat DAN cDNA as a probe. They determined that the DAN gene encodes a transcription factor homologous to a mouse tumor suppressor gene.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.