RANk (Receptor Activator Of Nuclear Factor Kappa B, CD265, TNFRSF11A, TNFRSF11-A, ODFR, OFE, TRANCER, Osteoclast differentiation factor receptor, Tumor Necrosis Factor Receptor Superfamily,Member 11a,NFKB Activator)

Antibodies / Antibodies-RANK

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Val330-Lys615 of human RANk, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.

RANk is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Studies of the mouse counterpart suggest that this receptor directly mediates the osteoprotegerin ligand (OPGL)-induced osteoclastogenesis in osteoclast precursor cells. Northern blot analysis indicated that the 4.5-kb human RANK mRNA is expressed ubiquitously. In some human cell lines, the authors detected additional transcripts that were derived from the use of alternative polyadenylation signals in the RANK gene.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- V SKTEIEEDSF RQMPTEDEYM DRPSQPTDQL LFLTEPGSKS TPPFSEPLEV GENDSLSQCF TGTQSTVGSE SCNCTEPLCR TDWTPMSSEN YLQKEVDSGH CPHWAASPSP NWADVCTGCR NPPGEDCEPL VGSPKRGPLP QCAYGMGLPP EEEASRTEAR DQPEDGADGR LPSSARAGAG SGSSPGGQSP ASGNVTGNSN STFISSGQVM NFKGDIIVVY VSQTSQEGAA AAAEPMGRPV QEETLARRDS FAGNGPRFPD PCGGPEGLRE PEKASRPVQE QGGAK

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.