SNRPD1 (Small Nuclear Ribonucleoprotein Polypeptide D1, SMD1, SNRP-D1, Sm-D autoantigen, snRNP core protein D1)

Antibodies / Antibodies-Nuclear Proteins

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Met1-Arg119 of human SNRPD1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.

Small nuclear ribonucleoprotein Sm D1 is a small nuclear ribonucleoprotein that belongs to the SNRNP core protein family. The protein may act as a charged protein scaffold to promote SNRNP assembly or strengthen SNRNP-SNRNP interactions through nonspecific electrostatic contacts with RNA. A clone was obtained that encoded a predicted 357aa protein with 9 Gly-Arg repeats at its carboxy terminus similar to regions in the Epstein-Barr virus nuclear proteins EBNA-1 and EBNA-2, as well as the yeast transcription factor SNF2. Lehmeier et al. (1994) showed that the D1 amino acid sequence is 29% identical to D3 and less than 15% identical to D2. The authors also showed that sera from 3 patients with lupus erythematosus detected the D1 and D3 components twice and the D2 component 3 times.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunocytochemistry: 1:50-500
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Optimal dilutions to be determined by the researcher.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MKLVRFLMKL SHETVTIELK NGTQVHGTIT GVDVSMNTHL KAVKMTLKNR EPVQLETLSI RGNNIRYFIL PDSLPLDTLL VDVEPKVKSK KREAVAGRGR GRGRGRGRGR GRGRGGPRR

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.