TMPRSS2 (Transmembrane Protease, Serine 2, PRSS10, Serine protease 10)

Antibodies / Antibodies-Enzymes, Protease (Proteinase, Peptidase)

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Ile254-Ser490 of mouse TMPRSS2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.

Transmembrane protease, serine 2 protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes.
This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.