VDAC1 (Voltage Dependent Anion Channel Protein 1, PORIN-31-HL, Porin 31HM, Plasmalemmal porin, Outer mitochondrial membrane protein porin 1, Voltage-dependent anion-selective channel protein 1)

Antibodies / Antibodies-Ion Channel

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Ala2-Ala283 of human VDAC1 fused to His-Tag and GST-tag at N-terminal expressed in E. coli.

The voltage-dependent anion channel (VDAC) of the outer mitochondrial membrane is a small, abundant outer membrane pore-forming protein found in the outer membranes of all eukaryotic mitochondria. The VDAC protein is thought to form the major pathway for movement of adenine nucleotides through the outer membrane and to be the mitochondrial binding site for hexokinase and glycerol kinase. Mitochondria expressing VDAC1 were capable of specifically binding hexokinase, whereas mitochondria expressing VDAC2 only bound hexokinase at background levels. Each human cDNA was expressed in essentially all human cell lines and tissues examined.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.