DDR1 (CD167a, Epithelial Discoidin Domain-containing Receptor 1, CD167 Antigen-like Family Member A, Cell Adhesion Kinase, Discoidin Receptor Tyrosine Kinase, Epithelial Discoidin Domain Receptor 1, HGK2, Mammary Carcinoma Kinase 10, MCK-10, Pro

Antibodies / Antibodies-Receptors

-20°C

Supplied as a liquid in PBS. No preservative added.

Recombinant protein corresponding to human DDR1.

Epithelial discoidin domain-containing receptor 1 (EC 2.7.10.1; UniProt Q08345; also known as CD167 antigen-like family member A, CD167a, Cell adhesion kinase, Discoidin receptor tyrosine kinase, Epithelial discoidin domain receptor 1, HGK2, Mammary carcinoma kinase 10, MCK-10, Protein-tyrosine kinase 3A, Protein-tyrosine kinase RTK-6, TRK E, Tyrosine kinase DDR, Tyrosine-protein kinase CAK) is encoded by the DDR1 (also known as CAK, EDDR1, MCK10, NEP, NTRK4, PTK3A, RTK6, TRKE) gene (Gene ID 780) in human. Discoidin domain-containing receptors (DDRs) belong to the superfamily of transmembrane receptor tyrosine kinases (RTKs). DDRs are distinguished from other RTKs by their extracellular discoidin motif and their utilization of collagens as internal ligands. While DDR1 binds essentially all types of collagens, DDR2 shows a preference for type I, II and III fibrillar collagens, and nonfibrillar type X collagen. DDR1 is widely expressed in epithelial cells in lung, kidney, colon, and brain, whereas DDR2 is primarily expressed in mesenchymal cells including fibroblasts, myofibroblasts, smooth muscle cells, and chondrocytes in kidney, skin, lung, heart, and connective tissues. DDRs are important mediators of fundamental cellular processes, such as proliferation, survival, differentiation, adhesion and matrix remodeling, their dysregulations are reported to cause a number of human diseases, including fibrotic disorders, atherosclerosis and cancer. DDR1 is initially produced with a signal peptide sequence (aa1-20), the removal of which yields the mature protein with an extracellular region (aa21-417) that contains the Discoidin domain (F5/8 type C domain or C2-like domain) at the N-terminal half (aa31-185) and a collagen-binding site within the membrane-distal DS domain (aa192-367), followed by a transmembrane segment (aa418-438) and a cytoplasmic region (aa439-913) that harbors a WW domain-interacting PPxY motif (aa481-484) and the kinase domain (aa610-905). Alternative splicings produce five isoforms, isoforms 2 (CAK II, DDR1a, Short) and 5 are expressed with a truncated cytoplasmic domain, while isoform 3 (DDR1d) is expressed in a 243aa soluble form and isoform 4 is expressed with a 6aa insertion (SFSLFS) between Ala665 and Arg666 of the canonical isoform 1 (CAK I, DDR1b) sequence.

Applications:
Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 10ug/ml detects CD167a/DDR1 on the surface of 3% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized human neonatal foreskin keratinocytes
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.