CD68 (Macrophage Antigen CD68, Macrosialin, Scavenger Receptor Class D Member 1, GP110, LAMP4, SCARD1)

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a liquid in PBS, 0.05% sodium azide.

Recombinant protein fragment corresponding to aa42-155 of human CD68 expressed in E. coli.

This gene encodes a 110kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms.

Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:200-1:400
ELISA: 1:10, 000
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:200-1:1000
Immunocytochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.