NET1 (Neuroepithelial Cell Transforming Gene 1, ARHGEF8, NET1A, Proto-oncogene p65 Net1, Rho Guanine Nucleotide Exchange Factor 8)

Pab

Blue Ice

Affinity Purified

30021010

By expression cloning and PCR of a neuroepithelioma cell line, followed by screening of a mammary epithelial cell line, Chan et al. (1996) obtained a cDNA encoding NET1. Sequence analysis predicted that the 601aa protein has a DBL homology (DH) domain that is 21% similar to that of DBL, particularly in the leucine-rich region. The DH domain is a 240aa region initially identified in DBL. DH domain proteins interact with members of the RAS superfamily of GTP-binding proteins. NET1 also possesses a pleckstrin-homology domain. Northern blot analysis revealed ubiquitous expression of major 3.0- and minor 2.4-kb NET1 transcripts, with highest levels in placenta. Functional analysis showed that NET1 induced morphologic alterations and conferred a malignant phenotype in vitro and in nude mice.

Applications: 
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.