Carcinoembryonic Antigen (CEA, CD66e) (Glycerol Free) (FITC)

Antibodies / Antibodies-Cancer Markers

-20°C

Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Fluorescein isothiocyanate (FITC).

Purified CEA from pooled tumors.

Carcinoembryonic Antigen (CEA) is a 180kD tumor-associated cell surface glycoprotein. CEA is synthesized during development in the fetal gut. It is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Anti-CEA is reportedly useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).

Applications:
Suitable for use in FLISA and Radiolabeling. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Hybridization of P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.