Carcinoembryonic Antigen (CEA, CD66e) (HRP)

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).

Human CD66e

Applications:
Suitable for use in Flow Cytometry (Not Tested), ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen/paraffin): 1:1000, requires protein digestion pre-treatment of paraffin sections.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse P3NSI myeloma cell line.

Recommended Secondary Antibodies:
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.